TABLE 1.
Chaperone expression plasmids constructed for this studya
| Plasmid | Promoter (chaperone genes)b | Regulatory gene | Drug resistancec |
|---|---|---|---|
| pKJE5 | trpp (dnaK-dnaJ-grpE) | trpR | Kmr |
| pKJE7 | araBp (dnaK-dnaJ-grpE) | araC | Cmr |
| pKJE8 | araBp (dnaK-dnaJ-grpE) | araC | Kmr |
| pGro6 | trpp (groES-groEL) | trpR | Kmr |
| pGro7 | araBp (groES-groEL) | araC | Cmr |
| pGro11 | Pzt-1p (groES-groEL) | tetR | Cmr |
| pGro12 | araBp (groES-groEL) | araC | Kmr |
| pG-KJE2d | trpp (dnaK-dnaJ-grpE) | trpR | Cmr |
| araBp (groES-groEL) | araC | ||
| pG-KJE3 | araBp (groES-groEL-dnaK-dnaJ-grpE) | araC | Cmr |
| pG-KJE6 | araBp (dnaK-dnaJ-grpE) | araC | Cmr |
| Pzt-1p (groES-groEL) | tetR | ||
| pG-KJE7 | araBp (groES-groEL-dnaK-dnaJ-grpE) | araC | Kmr |
a
All plasmids contained the replication origin of pACYC184 and genes encoding chaperones under control of the promoters indicated.
b
The chaperone genes (starting with the promoter-proximal gene) controlled by a given promoter are indicated in parentheses.
c
Kmr, kanamycin resistance; Cmr, chloramphenicol resistance.
d
The level of dnaK-dnaJ-grpE expression is low for unknown reasons.