TABLE 1.
Medium | Detection method | MPN · ml−1 on:
|
|||||
---|---|---|---|---|---|---|---|
Day 2 | Day 5 | Day 8 | Day 11 | Day 14 | Day 34 | ||
Sediment medium | Tracera | 2.3 × 101 (a)c | 9.3 × 102 (a) | 2.1 × 104 (a) | 2.1 × 104 (a) | 1.4 × 106 (a) | 1.3 × 107 (a) |
API-RST medium | Blackeningb | 4.3 × 103 (b) | 4.3 × 103 (a) | 4.3 × 103 (a) | 4.3 × 103 (a) | 4.3 × 103 (b) | 4.3 × 103 (b) |
Positive tubes were detected by the 35SO42− T-MPN method, and the detection limit was 0.1% TRIS.
Positive tubes were visually detected by the N-MPN method, i.e., by detection of a black precipitate (FeS).
MPN values in the same column followed by the same letter are not significantly different as determined by the Student t test (P > 0.05).