Fig 1. Experimental design and routes used for challenge.

In this study 10-week-old female CD-1 mice were challenged with B. pertussis and euthanized at 1 hour, 3 days, or 7 days post-challenge. The lungs, trachea, and nasal lavage fluid were collected to enumerate bacterial burden at each time point. Intranasal administration of B. pertussis was compared to aerosol challenge using a range of challenge doses.