Fig 1. Schematic of experimental design.

(A) Genetically divergent mouse strains BL6, A/J, and NOD were exposed to either the American diet or a standard mouse chow. Phenotypic measurements were collected and WGBS and RNA-seq were performed on DNA and RNA extracted from liver. Arrows represent differences from the American diet vs. standard diet comparison, as reported in [7]. (B) BL6 and NOD mouse strains were exposed to an American diet and were treated with the FXR agonist GW4064 or control vehicle. Phenotypic measurements were collected and WGBS and RNA-seq were performed on DNA and RNA extracted from liver. Arrows represent differences from the American diet + GW4064 vs. standard diet + vehicle comparison. Graphics were created with BioRender.com.