TABLE 1.
Characteristics of toluene-degrading strains T103 and T104
| Characteristic | Result for straina
|
|
|---|---|---|
| T103 | T104 | |
| Morphology | Rod | Rod |
| Flagella | − | − |
| Pigmentation | Yellow | Yellow |
| Gram stain | + | + |
| Acid fast | + | + |
| Nitrate reduction | − | − |
| Catalase | + | + |
| Oxidase | − | − |
| Citrate | + | + |
| Growth on sugars | ||
| Glucose | + | + |
| Lactose | − | − |
| Maltose | + | + |
| Sucrose | − | − |
| Growth on aromatic compoundsb | ||
| Toluene | + | + |
| Benzene | − | − |
| Chlorobenzene | − | − |
| o-Xylene | − | ++ |
| m-Xylene | − | ++ |
| p-Xylene | − | ++ |
| Phenol | − | − |
a
+, good growth or activity; −, poor or no growth or activity.
b
For growth on aromatic compounds, cultures were streaked onto agar plates containing a minimal salts media. Plates were then incubated in desiccators with substrate concentrations of approximately 10 mg/liter. The incubation temperature was 20°C. Each compound was tested twice. ++, very good growth within 3 days; +, good growth after 3 days; −, poor or no growth.