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. 2023 Nov 2;13:18914. doi: 10.1038/s41598-023-45978-4

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Relative expression levels of TmTak1 mRNA in developmental stages and tissues of T. molitor. (A) Expression levels of TmTak1 in T. molitor at the egg, the young larvae (YL), late larvae (LL), pre-pupae (PP), 1–7-day-old pupae (P1–7), and 1–5-day-old adult (A1–5) stages. Distribution of TmTak1 transcripts in larva and adult tissues. Fat bodies (FB), gut (GT), hemocytes (HC), integument (INT), and Malpighian tubules (MT) of (B) late instar larvae and (C) adults, in addition to ovaries (OV) and testes (TT) of adults, were dissected and collected from 20 late larvae and 5-day-old adults for analysis. T. molitor 60S ribosomal protein L27a (TmL27a)-encoding gene was used as an internal control.