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. 2023 Nov 2;13:18914. doi: 10.1038/s41598-023-45978-4

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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TmTak1 mRNA expression profiles after microbial challenge. Expression of TmTak1 mRNA in the whole body (A), hemocytes (B), gut (C), fat bodies (D), Malpighian tubules (E), integument (F) of larvae infected with Escherichia coli, Staphylococcus aureus, and Candida albicans. The expression was analyzed by qPCR using L27a (T. molitor) as the internal control. For each time point, the expression level in the phosphate-buffered saline (PBS)-injected control (mock control) was set to 1; this is represented by a dotted line. vertical bars represent mean ± standard error from three biological replicates.