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. 2023 Nov 2;13:18914. doi: 10.1038/s41598-023-45978-4

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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AMPs mRNA expression levels in the hemocytes against E. coli, S. aureus, and C. albicans after TmTak1 knockdown. To evaluate the functional properties of TmTak1 in regulating AMP genes expression in response to pathogens, we used dsTmTak1, the amount of AMP expression was examined 24 h after systemic infection. AMP genes, including TmTene1 (A), TmTene2 (B), TmTene3 (C), TmTene4 (D), TmDef (E), TmDef-like (F), TmCec2 (G), TmColeA (H), TmColeB (I), TmColeC (J), TmAtt1a (K), TmAtt1b (L), TmAtt2 (M), TmTLP1 (N), and TmTLP2 (O).