TABLE 1.

PCR primers and oligonucleotide probes used for IPC construction and detection of Giardia, Cryptosporidium, and IPC

Oligonucleotide		Target	Sequence	PCR product size (bp)	Reference(s)
Primer pair or probe	Name
Primer pair 1	GGL	Giardia giardin gene	5′-AAGTGCGTCAACGAGCAGCT-3′	171	23, 24
	GGR		5′-TTAGTGCTTTGTGACCATCGA-3′
Primer pair 2	CHSP1	C. parvum hsp70 gene	5′-AGCAATCCTCTGCCGTACAGG-3′	590	39
	CHSP4		5′-AAGAGCATCCTTGATCTTCT-3′
Primer pair 3	IPCF	IPC	5′-ATGACAGCCACTCCT-3′	290	This study
	IPCR		5′-ATGTCAGTTGTGACCACGAA-3′
Primer pair 4	GHSP1	Giardia heat shock gene	5′-AGGGCTCCGGCATAACTTTCC-3′	163	6
	GHSP2		5′-GTATCTGTGACCCGTCCGAG-3′
Primer pair 5	CPHSP2423	Cryptosporidium spp. hsp70 gene	5′-AAATGGTGAGCAATCCTCTG-3′	307	29
	CPHSP2764		5′-CTTGCTGCTCTTACCAGTAC-3′
Primer pair 6	T7 promoter-CHSP1 link	IPC	5′-GGATCCTAATACGACTCACTATAATGACAGCCACTCCT-3′	328	This study
	Poly(T)15-CHSP4 link		5′-TTTTTTTTTTTTTTTATGTCAGTTGTGACCACGAA-3′
Probe 1	GGP	GGL-GGR PCR product	5′-TCGAGGACGTCGTCTCGAAGATCCAG-3′		23, 24
Probe 2	CPHSP2475	CPHSP2423-CPHSP2764 PCR product	5′CCATTATCACTCGGTTTAGA-3′		29