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Comparison of IF-DAPI-PI microscopy with RT-PCR for detection of viable Giardia and Cryptosporidium in nonspiked water sample concentrates
| Sample | n | Vol analyzed (liters) | No. of samples positive by:
|
|||
|---|---|---|---|---|---|---|
| IF microscopya
|
RT-PCR
|
|||||
| Giardia spp. | Cryptosporidium | Giardia spp. | C. parvum | |||
| Treated sewage discharge | 6 | 10 | 3 (1–2) | 0 | 4 | 0 |
| River or creek water | 19 | 2–110 | 4 (1) | 4 (1–3) | 16 | 1 |
| Treated drinking water | 4 | 32–300 | 0 | 0 | 0 | 0 |
Numbers in parentheses are the count ranges per volumes analyzed.
