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. 2023 Nov 2;8:169. doi: 10.1038/s41541-023-00766-z

Fig. 5. Experimental design and biodistribution of PEGylated LNP in representative organs of rat.

Fig. 5

a Schematic illustration of the experimental protocols. Wistar rats were injected intramuscularly with 0.009 (L-LNP group), 0.342 (M-LNP group), or 2.358 (H-LNP group) mg phospholipids/kg DiR-LU@LNP on Day 0 and Day 21, respectively. Rats in the Control group were injected with PBS. Six hours after each injection, three rats from each experimental group were sacrificed and immediately dissected. Major organs including heart, liver, spleen, lung, kidneys and draining lymph node, and muscle at the injection site were collected for fluorescence imaging with IVIS Spectrum imaging system. b Representative fluorescence images of major organs and muscle tissues isolated from rats 6 h after the first and second injection of DiR-LU@LNP. c Total radiant efficiency of major organs determined 6 h after the first and second injection of DiR-LU@LNP. Data were presented as “mean ± standard deviation” (n = 3). Differences in total radiation efficiency induced by three doses were analyzed using multiple unpaired t tests with correction for multiple testing. *P < 0.05; **P < 0.01; ***P < 0.001; ****P < 0.0001.