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. 2023 Nov 2;14:7000. doi: 10.1038/s41467-023-42669-6

Fig. 4. Viral assembly in glycoengineered cells.

Fig. 4

a HaCaT cells grown on cover slips were infected with MOI10 of HSV-1 K26-GFP and fixed and permeabilized at 14 hpi followed by co-staining for HSV-1 gD (magenta) and E-cadherin (cyan). Histograms on the left indicate intensities of gD and E-cadherin signals across the confocal images (marked with black arrowheads). Pixel overlap from the two channels is shown in white. GFP labeled capsid proteins (VP26) are seen in green. Nuclei were stained with DAPI (blue). Stainings of mock-infected cells are included. Scale bar: 10 µm. Images are representative of two independent experiments. b HaCaT cells grown on cover slips were infected with MOI10 of HSV-1 K26-GFP and fixed and permeabilized at 14 hpi followed by staining for HSV-1 gB (magenta). GFP labeled capsid proteins (VP26) are seen in green. Nuclei were stained with DAPI (blue). Scale bar: 10 µm. Magnified regions of merged images are indicated with dashed white boxes. Images are representative of 2 independent experiments. c HaCaT WT and C1GALT1C1 KO cells were infected with MOI3 or MOI10 of HSV-1 K26-GFP and viral capsids imaged by live microscopy at 14 and 20 hpi. Fluorescent images overlaid with bright field images are also shown. Scale bar: 10 µm. Images are representative of two independent experiments.