Fig. 4. CC-GEMS signaling can be rerouted through the alternative PLCG pathway.

a Schematic overview of the reporter system to monitor CC-GEMS receptor activation using phospholipase C gamma (PLCG) intracellular signaling. HEK293T cells were transfected with A-type_PLCG receptors with linker α₂ and a SEAP reporter gene, engineered to be responsive to PLCG activation, through an NFAT (nuclear factor of activated T cells)-responsive minimal promoter (“Methods”). Cells were subsequently treated with 0.12 μM SUMO-tagged A’-A’ with l₂. b SEAP activity [U/L], measured 48 hours following transfection (see “Methods”). Fold change and significance (two-tailed, unpaired t test, P = 0.0002) is noted above bars. ns: P > 0.05, *P ≤ 0.05, **P ≤ 0.01, ***P ≤ 0.001 (see Supplementary Table S6). Bars indicate mean activity; individual data points represent independent triplicates, performed on the same day. Source data are provided as a Source Data file.