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. 2023 Nov 2;14:7001. doi: 10.1038/s41467-023-42810-5

Fig. 6. Engineering intercellular communication using CC-GEMS.

Fig. 6

a Intercellular communication is achieved by engineering sender HEK293S GnTi- TetR cells that, upon induction with doxycycline (dox), secrete SUMO-tagged ditopic A’-A’ ligand (see “Methods” and Supplementary Figs. S26 and  S28) and receiver HEK293T cells expressing the receptor, STAT3 and reporter gene SEAP (see Methods). b SEAP activity [U/L] in co-cultures of 1.5 × 105 receiver and 6 × 105 sender cells or control (untransduced HEK293S GnTi- TetR) following incubation with and without dox (see “Methods”). c AND gate logic is achieved by engineering sender 1 (see Supplementary Fig. S32) and sender 2 (see Supplementary Fig. S33) HEK293S GnTi− cells that secrete SUMO-tagged ditopic A’-Γ and Γ’-A’ ligands with l4 respectively (see “Methods”) and receiver HEK293T cells. d SEAP activity (in U/L) for AND gate logic, after incubation of 1.5 × 105 receivers with 5 × 105 senders 1, 5 × 105 senders 2 and/or control, (untransduced HEK293S GnTi- cells) for 48 hours (see “Methods”). Bars indicate mean activity; individual data points represent independent triplicates, performed on the same day. Significance (one-way ANOVA with Tukey’s multiple comparison test) and fold change is noted above the bars. ns: P > 0.05, *P ≤ 0.05, **P ≤ 0.01, ***P ≤ 0.001 (see Supplementary Table S4). Source data are provided as a Source Data file.