Fig. 1.
Effect of bimatoprost (Bim) and prostaglandin F2α ethanolamide (PGF2αEA) on 3T3-L1 preadipocyte viability. 3T3-L1 preadipocytes (A–C) were untreated or treated with vehicle DMSO (0.1%) or PD98059 (PD; 5 μM) or Bim (1 μM)/PGF2αEA (10 μM) without or with PD, for four days. The cell viability was estimated using the MTT assay. HepG2 (D) and HEK293T (E) cells were also untreated or treated with DMSO, Bim, or PGF2αEA for MTT assay after four days of treatment. The observed effects on cell viability in 3T3-L1 were further studied using the crystal violet cell viability assay (F) under the same treatment timetable. The results are expressed as percentages relative to the untreated control (MTT assay) or to vehicle control (crystal violet assay) and are illustrated as the mean ± SD, for n = 4 and n = 3 independent bioreplicates, respectively. ∗P < 0.05, ∗∗P < 0.01. MTT, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide.