Fig. 2.
Effect of bimatoprost (Bim) and prostaglandin F2α ethanolamide (PGF2αEA) on 3T3-L1 preadipocyte cell number. 3T3-L1 cells were untreated or treated with DMSO (0.1%) or Bim (1 μM)/PGF2αEA (10 μM), without or with PD98059 (PD; 5 μM), or PD, for four days, and then counted with a hemocytometer (A). HepG2 (B) and HEK293T (C) cells were also untreated or treated with DMSO, Bim, or PGF2αEA, for four days and counted as for 3T3-L1. In addition, a BrdU incorporation cell proliferation ELISA was performed on 3T3-L1 cells that were plated and treated as indicated in (A), with DMSO, Bim, or PGF2αEA besides the assay blank (no cells) and background control (cells treated with DMSO, but without BrdU addition; No BrdU), for two days. The colorimetrically measured average BrdU incorporation percentages to DMSO control are presented (D). The results are shown as the mean ± SD of three independent bioreplicates. ∗∗P < 0.01, ∗∗∗∗P < 0.0001. BrdU, 5-bromo-2′-deoxyuridine.