Fig. 3.
Effect of bimatoprost (Bim) and prostaglandin F2α ethanolamide (PGF2αEA) on 3T3-L1 preadipocyte cell cycle. 3T3-L1 cells were either untreated or treated with DMSO (0.1%) or PD98059 (PD; 5 μM) or with Bim (1 μM)/ PGF2αEA (10 μM), without or with PD, for two days. The percentage of cells in the G1, S, and G2 cell cycle phases was evaluated by flow cytometry. A representative image of flow cytometry histograms for all treatments is shown in Fig. 3, along with quantification of the cell percentage within the sum of the S and G2 cell cycle phases (Fig. 3 bar chart). The results are shown as the mean ± SD of three independent bioreplicates. ∗P < 0.05.