Fig. 4.
Effect of bimatoprost (Bim) and prostaglandin F2α ethanolamide (PGF2αEA) on 3T3-L1 preadipocytes as per microscopic observation and caspase activity assay. 3T3-L1 cells were treated with DMSO (0.1%), Bim (1 μM), PGF2αEA (10 μM), or camptothecin (CPT; 1 μM) as a positive control for apoptosis, for two days, after which the cells were photographed under the setting of 10X magnification of an inverted microscope. Representative images of treated cells from each of the three bioreplicates are illustrated (A). Scale bar indicates 75 μm. The total number of cells per plate, as counted with a hemocytometer, was determined (B). Caspase activity, relative to DMSO vehicle control, was determined for both Bim and PGF2αEA (C). The results are shown as the mean ± SD of three independent bioreplicates. ∗∗P < 0.01, ∗∗∗P < 0.001.