Fig. 5.
Effect of bimatoprost (Bim) and prostaglandin F2α ethanolamide (PGF2αEA) on 3T3-L1 cell cycle cyclin-dependent kinase (CDK) and CDK inhibitor (CDKI) gene expression. 3T3-L1 cells were untreated or treated with DMSO (0.1%), PD98059 (PD; 5 μM), or Bim (1 μM)/ PGF2αEA (10 μM), without or with PD, for two days, and then the gene expression of the CDKIs Cdkn1a, Cdkn1b, and CDKs Cdk1, 2, 4, and 5 was quantified by quantitative PCR. The results are shown as the mean ± SD of relative gene expression to the untreated control, for three independent bioreplicates. ∗P < 0.05, ∗∗P < 0.01, ∗∗∗P < 0.001, ∗∗∗∗P < 0.0001.