Fig. 6.
Effect of bimatoprost (Bim) and prostaglandin F2α ethanolamide (PGF2αEA) on peroxisome proliferator-activated receptor gamma (PPARG) activity. HEK293T cells were transfected with a PPARG expression construct, the peroxisome proliferator response element (PPRE)-luciferase reporter construct, and constitutively active β-galactosidase plasmid construct. Then, one day posttransfection, the cells were untreated or treated with DMSO vehicle (0.1%) or PD98059 (PD; 5 μM), or Bim (1 μM)/PGF2αEA (10 μM) without or with PD, followed by rosiglitazone (Rosi; 0.01 μM) treatment. After 24 h, the luminescence was read and corrected to β-galactosidase activity. The results are depicted as the mean ± SD of relative luminescence units (RLU) presented as percentages to the untreated control, for three independent bioreplicates. ∗P < 0.05, ∗∗P < 0.01, ∗∗∗P < 0.001, ∗∗∗∗P < 0.0001.