Effect of bimatoprost (Bim) and prostaglandin F2α ethanolamide (PGF2αEA) on differentiating 3T3-L1 cells. 3T3-L1 preadipocytes were induced to differentiate (Diff.) or not (Undiff.). In both cases, the cells were untreated or treated with either DMSO (0.1%), Bim (1 μM), or PGF2αEA (10 μM), for two days. The cell viability was then quantified with the MTT assay. The results are presented as percentages relative to the untreated undifferentiated control and are illustrated as the mean ± SD of four independent bioreplicates. ∗P < 0.05, ∗∗P < 0.01, ∗∗∗P < 0.001, ∗∗∗∗P < 0.0001. MTT, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide.