Table 1.
Primer sequences for quantitative PCR
| Gene Name | Abbreviation | Common Name | Reverse Primer (5'-3') Forward Primer (5'-3') |
|---|---|---|---|
| Cyclin-dependent kinase inhibitor 1A | Cdkn1a | p21 | CTTGTCGCTGTCTTGCACT AATCTGCGCTTGGAGTGATAG |
| Cyclin-dependent kinase inhibitor 1B | Cdkn1b | p27 | GAGCAGACGCCCAAGAAG GCAGTGATGTATCTAATAAACAAGGA |
| Transformation-related protein 53 | Trp53 | p53 | CAGGGAGCGCAAAGAGAG CTCCCGGAACATCTCGAAG |
| Cyclin-dependent kinase 1 | Cdk1 | Cdk1 | GACTACAAGAACACCTTTCCCA CGTTTGGCAGGATCATAGACT |
| Cyclin-dependent kinase 2 | Cdk2 | Cdk2 | GCCTGATTATAAGCCAAGTTTCC GTCATAGTGCAGCATTTGCG |
| Cyclin-dependent kinase 4 | Cdk4 | Cdk4 | GGCTGAAATTGGTGTCGGT CTCACGAACTGTGCTGACG |
| Cyclin-dependent kinase 5 | Cdk5 | Cdk5 | CACCGACTGAGGAACAATGG GGTTACACTTCAATAGGTTCTGC |
| TATA box-binding protein∗ | Tbp | Tbp | TGTATCTACCGTGAATCTTGGC CCAGAACTGAAAATCAACGCAG |
| Hypoxanthine guanine phosphoribosyl transferase∗ | Hprt | Hprt | CCCCAAAATGGTTAAGGTTGC AACAAAGTCTGGCCTGTATCC |
| Glyceraldehyde-3-phosphate dehydrogenase∗ | Gapdh | Gapdh | AATGGTGAAGGTCGGTGTG GTGGAGTCATACTGGAACATGTAG |
∗
Housekeeping gene for data normalization.