Fig. 8.

MSC Chemotaxis induced by the decellularized cartilage gel. Representative time course images of collagen gel-embedded cells in response to decellularized cartilage ECM immediately after seeding (A), at 12 h (B) and 24 h (C) with overlaid cell migration trajectories. (D) 3D chemotaxis assays with live cell imaging. Migration tracks of human MSCs in response to (E–L) SDF-1alpha, (M–P) dcECM P2. Forward migration index (FMI) parallel to the gradient of hMSCs migrating in response to SDF-1alpha and dcECM (Q) (n = 40) and the center of mass of cellular endpoints from trajectory plots (R). Adapted from the study by Golebiowska et al. with permission of Annals of Biomedical Engineering, copyright 2023 [164].