Table 3.

Primer sets designed for the recovery of Cryptosporidium aDNA targeting different regions of its 18S gene.

Gene Target	Primer name	Primer seq.	Amplicon size (bp)	References
18S	Nem18SlongF Nem18SlongR	5′-CAGGGCAAGTCTGGTGCCAGCAGC-3′ 5′-GACTTTCGTTCTTGATTAATGAA-3′	350–400	Wood et al. (2013)
18S	–	5′-GCGAAACGATTTGCCAAGGA-3′ 5′-AGTTTCAGCCTTGCGACCAT-3′	195	Rollins et al. (2021)
18S	–	5′-GGAGCCTGCGGCTTAATTTG-3′ 5′-CCACCAACTAAGAACGGCCA-3′	127	Rollins et al. (2021)