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. 2023 Sep 4;299(10):105226. doi: 10.1016/j.jbc.2023.105226

Figure 7.

Figure 7

FKBP5 rescues Klf15KO mice from impaired muscle regeneration. TA muscles of WT and Klf15KO mice were injected with adenovirus expressing FKBP5 (Ad-FKBP5) or control adenovirus (Ad-Ctrl) at day 1 post-CTX injury and harvested at day 7 or day 14 after injury. A, immunostaining for MyHC (red) and laminin (green) in TA muscles at day 7. B, percentage of MyHC+ fibers per laminin+ myofiber were quantified (n = 6, ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001, one-way ANOVA). C, representative H&E-stained TA muscles at day 14 (n = 6). D, representative WGA-stained TA muscles illustrating the size of myofiber at day 14. E, quantification of average cross-sectional area (CSA) of regenerating myofibers at day 14 (n = 6, ∗p < 0.05, ∗∗p < 0.01, one-way ANOVA). F, Picro-Sirius red–stained TA muscles showed the collagen deposition at day 14. G, quantitative analysis of the area of positive Picro-Sirius red staining (n = 6, ∗∗∗p < 0.001, ns, no significant difference, one-way ANOVA). CTX, cardiotoxin; FKBP5, FK506-binding protein 51; KLF15, Krüppel-like factor 15.