Fig. 8.

Comparison of the distal heme environment in cytochrome cd₁ Nitrite Reductase, Mb F43H/H64A mutant and neuroglobin F28H/F42Y mutant. Left, Pseudomonas aeruginosa cyt cd₁NR; Middle, sperm whale F43H/H64 A Mb; Right, putative structure of the human Ngb F28H/F42Y mutant. Heme and selected amino acids are shown as sticks. Water molecules (oxygen atom) in the proximity of the heme iron are shown as red spheres. Ngb structure was generated with PyMOL 0.99rc6 using the mutagenesis option and selecting for the rotamer with less steric clashes. Figure was generated with PyMOL 0.99rc6 [26]. Protein Data Bank entries 1NIR (cyt cd₁NR), 5HLQ (Mb), and 1OJ6 (Ngb) were used.