Fig. S1.

Concentration-dependence and toxicity of clozapine in SW872 cells.

(A) SW872 cells were grown for 3 days with increasing concentration of CLZ. LDs were quantified by ORO spectrophotometric analysis at 510 nm. Data are expressed in % respect to T0 condition. Results represent the means ± SEM calculated from at least three independent determinations, **p < 0.01, as compared to T0; (*) p < 0.05 as compared to T3 (one-way ANOVA followed by Dunnett's multiple comparisons). (B) Flow cytometric evaluation of DNA content showing the absence of sub diploid peaks in cells grown for 3 days with or without CLZ (15 μM). (C) Statistical analysis of the results obtained by PI labelling of fresh cells, for general viability assessment. T3 CTR received DMSO alone. Results represent the means ± SEM calculated from at least three independent determinations. ns: not significant.