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CLZ does not exert any toxic effects in MEFs.
Confluent MEFs were induced to differentiate for 3 days with or without CLZ (15 μM). (A) Flow cytometric evaluation of DNA content showing absence of subdiploid peaks. (B) Statistical analysis of the results obtained by PI labelling of fresh cells, for general viability assessment. Results represent the means ± SEM calculated from at least three independent determinations; ns: not significant (Unpaired t-test).
