Fig. 4.

Neuronal activation following optogenetic stimulation in vDG of GRov mice. (A) The extent of neuronal activation in the brain following light stimulation in vDG. Relative to dDG, optogenetic activation of vDG led to an increased cFos mRNA expression in more brain regions. Representative images are shown ranging from Bregma 1.98 mm (indicated by the no. 1) through Bregma −3.52 mm (indicated by the no. 8). (B) Images were quantitatively analyzed. Two-way ANOVA analyses were performed on listed regions, followed by Šídák's multiple comparisons test. Two-way ANOVA revealed a significant genotype × light interaction in DG [F(1, 15) = 8.62, P < 0.05], in CA1 [F(1, 15) = 8.39, P < 0.05], in CA3 [F(1, 15) = 4.76, P < 0.05], in pBLA[F(1, 15) = 6.50, P < 0.05], in Ahi [F(1, 15) = 6.71, P < 0.05], and in PMCo [F(1, 15) = 7.12, P < 0.05]; a tendency to be significance in M2 [F(1, 15) = 4.31, P = 0.056], in LS [F(1, 15) = 3.08, P = 0.1], and in PVN [F(1, 15) = 3.17, P = 0.1]. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001 versus respective no stimulation group; ^#P < 0.05, ^##P < 0.01 versus WT group under the same condition. All listed regions showed increased cFos expression as a result of stimulation in vDG of GRov mice. vDG stimulation resulted in a greater degree of cFos response in M2, LS, DG, CA1, and CA3 of GRov mice. Activation in vDG of GRov resulted in an extensive cFos response in the basal posterior amygdaloid complex, including pBLA, AHi, and PMCo. Mean ± SEM. WT (light off), n = 4 mice; WT (light on), n = 5 mice; GRov (light off), n = 5 mice; GRov (light on), n = 5 mice.