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. 2023 Oct 16;27:100581. doi: 10.1016/j.ynstr.2023.100581

Fig. 6.

Fig. 6

Enhanced glutamatergic activation in the basal posterior amygdala of GRov mice following optogenetic activation of vDG. (A) Representative confocal images showing the cFos activity in pBLA, AHi, and PMCo following light stimulation in vDG of WT and GRov mice. Greater cFos neuronal density and a predominant colocalization with Vglut1+ (magenta) can be seen in GRov-stimulated group compared to WT-stimulated group. cFos (red), Vglut1 (blue), Gad2 (green), cFos + Vglut1 (magenta). Scale bars, 200 μm. (B) Magnified views of cFos (red) co-labeling with Vglut1+ (blue) show that the majority of activated neurons are Vglut1+. Representative cFos+Vglut1 colocalization is indicated by magenta arrows and neurons expressing cFos and Vglut1 are indicated by red and blue arrows, respectively. Scale bars, 50 μm. (C) Magnified views of cFos (red) co-labeling with Gad2+ (green) show fewer activated (yellow) neurons among the Gad2+ population. Representative cFos+Gad2 colocalization is indicated by yellow arrows and neurons expressing cFos and Gad2 are indicated by red and green arrows, respectively. Scale bars, 50 μm. (D) Histogram shows the percentage of cFos+Vglut1+ to total Vglut1+ neuronal number density in the pBLA, AHi, and PMCo following the optogenetic stimulation of vDG in both WT and GRov groups in comparison to the non-stimulated groups. Two-way ANOVA analyses were performed on listed regions, followed by Šídák's multiple comparisons test. Two-way ANOVA revealed a significant genotype × light interaction in pBLA [F(1, 11) = 18.36, P < 0.01], in AHi [F(1, 11) = 25.18, P < 0.01], and in PMCo [F(1, 11) = 7.64, P < 0.05]. *P < 0.05, **P < 0.01, ****P < 0.0001 versus respective no stimulation group; ##P < 0.01, ####P < 0.0001 versus the stimulated WT group. There was no genotype difference in cFos-based glutamatergic activity in pBLA, AHi, and PMCo between GRov and WT mice under the no stimulation condition. Significantly higher cFos-based glutamatergic activity was observed in pBLA, AHi and PMCo of the stimulated GRov group compared to the stimulated WT group. (E) Histogram shows the percentage of cFos+Gad2+ to total Gad2+ neuronal number density in the pBLA, AHi, and PMCo following the optogenetic stimulation of vDG in both GRov and WT groups compared to the no stimulation groups. Two-way ANOVA analyses were performed on listed regions, followed by Šídák's multiple comparisons test. **P < 0.01, ***P < 0.001, ****P < 0.0001 versus respective no stimulation group. There was no genotype difference in cFos-based GABAergic activity in pBLA, AHi, and PMCo between GRov and WT mice either basally or under the stimulation condition. Mean ± SEM. WT (light off), n = 3 mice; GRov (light off), n = 3 mice; WT (light on), n = 4 mice; GRov (light on), n = 5 mice.