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. Author manuscript; available in PMC: 2024 Sep 20.
Published in final edited form as: J Am Chem Soc. 2023 Aug 30;145(37):20189–20195. doi: 10.1021/jacs.3c07937

Figure 3. Decaging of fluorosulfate-containing peptides and proteins under physiologically relevant conditions.

Figure 3.

a) Fluorosulfate decaging in fsY-containing synthetic peptides. b) Light-mediated fluorosulfate decaging in C5aR1 22mer peptide using photocaged reagent 20. c) TTI peptide sequences and sulfation patterns. d) Thrombin inhibition assay of TTI peptides. Data were fitted to the Morrison inhibition model and error bars represent the standard deviation of three independent measurements. e) Light-mediated activation and decaging of fluorosulfate-containing TTI peptide TTI04(fsY) regulates its sulfation-dependent thrombin inhibitory activity. f) Fluorosulfate decaging in fsY-containing protein sfGFP-151-fsY and its corresponding high-resolution mass spectrometry.50