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. Author manuscript; available in PMC: 2024 Sep 20.
Published in final edited form as: J Am Chem Soc. 2023 Aug 30;145(37):20189–20195. doi: 10.1021/jacs.3c07937

Figure 4. Cytocompatibility of the reagents.

Figure 4.

a) Sortase A-mediated ligation of peptide 22 onto the S. aureus cell surface and its decaging followed by the TEV protease cleavage. b) Percent of S. aureus cell survived after sortase A-mediated ligation of 22 (Step I) and after fluorosulfate decaging by 7 (Step II) compared to the cells treated with PBS. The average data of two trials were plotted. c) LC-MS analysis of samples after the TEV cleavage identified the decaged peptide (24, bottom) compared to the cleaved peptide before decaging (23, top). d) MTT assay of the mammalian HEK-293T cells after incubation with various concentrations of reagents 5, 6, and 7. The average data of three trials were plotted.