Figure 13.

Heterogeneity of hypoxia in hind limb SQ20b human squamous cell carcinoma subcutaneous xenograft in mice shown by three-color hypoxia imaging. Blue is Hoechst 33342 (nuclei), green is pimonidazole (hypoxia marker 1), and red is carbonic anhydrase (hypoxia marker 2). Left bar is 2 mm, right bar is 500 μm. The right image shows high magnification of the region of interest shown on the left by a white rectangle. The circular hole was caused by angiocatheter placement before tumor sectioning. This research was originally published in ref (135). Copyright 2014 Society of Nuclear Medicine and Molecular Imaging, Inc.