TABLE 1.
Estimated copy number of RSα and RSβ and the mean generation time in B. japonicum HRS and normal isolates
| Isolate or strain | Estimated copy numbera
|
Mean generation time (h) | |
|---|---|---|---|
| RSα | RSβ | ||
| Normal isolates | |||
| NC4a | 8 | 9 | 5.8 |
| NC6a | 9 | 6 | 6.9 |
| NC41a | 7 | 7 | 5.8 |
| NK2 | 7 | 8 | 6.6 |
| NK8 | 9 | 9 | 6.0 |
| NK23 | 7 | 7 | 6.6 |
| T7 | 5 | 3 | 7.6 |
| T9 | 7 | 2 | 5.9 |
| T12 | 7 | 5 | 7.2 |
| Mean ± SD | 7.3 ± 1.2 | 6.2 ± 2.5 | 6.5 ± 0.7 |
| Niigata-type HRS isolates | |||
| NC3a | 175 | 25 | 8.9 |
| NC32a | 131 | 34 | 10.1 |
| NK4 | 144 | 38 | 10.3 |
| NK5 | 171 | 45 | 11.2 |
| NK6 | 118 | 22 | 9.9 |
| NK9 | 115 | 23 | 10.0 |
| NK10 | 132 | 38 | 10.8 |
| NK16 | 99 | 23 | 11.2 |
| NK18 | 106 | 24 | 9.5 |
| NK20 | 150 | 29 | 10.4 |
| NK25 | 105 | 29 | 10.5 |
| NK28b | 138 | 43 | 10.6 |
| NK29 | 124 | 44 | 10.8 |
| NK34 | 115 | 40 | 11.8 |
| NK37 | 86 | 27 | 9.1 |
| NK40 | 133 | 45 | 9.6 |
| Mean ± SD | 128 ± 25 | 33 ± 9 | 10.3 ± 0.8 |
| Tokachi-type HRS isolates | |||
| T2 | 19 | 41 | 8.3 |
| T15 | 22 | 46 | 10.1 |
| T22 | 17 | 51 | 13.1 |
| T25 | 22 | 47 | 10.1 |
| T31 | 23 | 35 | 8.6 |
| Mean ± SD | 21 ± 3 | 44 ± 6 | 10.0 ± 1.9 |
| USDA reference strains | |||
| USDA 110 | 12 | 6 | 6.2 |
| USDA 122 | 8 | 7 | 6.5 |
| USDA 123b | 23 | 42 | 8.5 |
a
Estimated copy numbers of HRS and normal isolates were determined based on the relative intensity and band number of RS-specific hybridization. To estimate the copy number of RSα and RSβ of HRS isolates and USDA 123, radioactivity was measured as described in the text. The band numbers of RS-specific hybridization of normal isolates and USDA 122 were counted.
b
Strain USDA 123 had copy numbers of RSα and RSβ comparable to those of the Tokachi-type HRS isolates.