Table 2.12.1.

Preparing the Kinase Mix^a

	Volume (each)	Volume (20×)
10× Kinase assay buffer (see recipe)	2 μl	40 μl
100 mM DTT	0.4 μl	8 μl
JNK3α2 (12 μM stock)	0.8 μl	16 μl
p-MKK4 or p-MKK7 (10 μM stocks)	0.1 μl	2 μl
Total	3.3 μl	66 μl

^aAntibodies: phospho-JNK antibody (rabbit, Cell Signaling Technology, cat. no. 9251); total JNK antibody (rabbit, Cell Signaling Technology, cat. no. 9252).