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. Author manuscript; available in PMC: 2024 Sep 1.
Published in final edited form as: Curr Protoc. 2023 Sep;3(9):e839. doi: 10.1002/cpz1.839

Table 2.12.2.

Preparing Arrestin-3 Solutions

Tube Number A1 A2 A3 A4 A5 A6 A7 A8 A9 A10 A11 A12 A13
Arrestin-3 concentration (μM) 0 0.1 0.2 0.4 0.6 1.2 2 4 8 12 20 30 45
Arrestin-3 (μl) 0 2a 4 8 12 2.4 4 8 2.4 3.6 6 9.2 13.2
Tris buffer (μl)b 14.7 12.7 10.7 6.7 2.7 12.3 10.7 6.7 12.3 11.1 8.7 5.5 1.5
a

Arrestin stock concentrations: For A2-A5, 1 μM; For A6-A8, 10 μM; For A9-A13, 65 μM.

b

Tris buffer: 10 mM Tris·Cl, pH 7.5, and 100 mM NaCl.