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Preparing Arrestin-3 Solutions
| Tube number | A1 | A2 | A3 | A4 | A5 | A6 | A7 |
|---|---|---|---|---|---|---|---|
| Arrestin-3 concentration (μM) | 0 | 0.2 | 0.5 | 1 | 5 | 10 | 20 |
| Arrestin-3 (μl)a | 0 | 4 | 10 | 1 | 5 | 10 | 10 |
| Tris buffer (μl) | 14.7 | 10.7 | 4.7 | 13.7 | 9.7 | 4.7 | 4.7 |
Arrestin stock concentrations: For A2-A3, 1 μM; For A4-A6, 20 μM; For A7, 40 μM.
