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. Author manuscript; available in PMC: 2024 Sep 1.
Published in final edited form as: Curr Protoc. 2023 Sep;3(9):e839. doi: 10.1002/cpz1.839

Table 2.12.6.

Transfection to Determine ASK1- and Arrestin-3-Dependent JNK Activation

pcDNA3-HA-ASK1a 0 μg 0.05 μg 0.1 μg 0.25 μg 0.5 μg 1 μg 1.5 μg
pcDNA3 1.5 μg 1.45 μg 1.4 μg 1.25 μg 1.0 μg 0.5 μg 0 μg
pcDNA3-arrestin-3 0.5 μg 0.5 μg 0.5 μg 0.5 μg 0.5 μg 0.5 μg 0.5 μg
FuGENE HD 6 μl 6 μl 6 μl 6 μl 6 μl 6 μl 6 μl
Opti-MEM 125 μl 125 μl 125 μl 125 μl 125 μl 125 μl 125 μl
a

For MKK7-induced JNK activation, add pcDNA3-Flag-MKK7β1 instead of pcDNA3-HA-ASK1.