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. Author manuscript; available in PMC: 2024 Sep 1.
Published in final edited form as: Curr Protoc. 2023 Sep;3(9):e839. doi: 10.1002/cpz1.839

Table 1.12.7.

Transfection to Determine MKK4-Dependent JNK Activation

pcDNA3-HA-MKK4 0 μg 0.2 μg 0.5 μg 1.0 μg 2.0 μg 4.0 μg
pcDNA3 4.5 μg 4.3 μg 4.0 μg 3.5 μg 2.5 μg 0.5 μg
FuGENE HD 13.5 μl 13.5 μl 13.5 μl 13.5 μl 13.5 μl 13.5 μl
Opti-MEM 125 μl 125 μl 125 μl 125 μl 125 μl 125 μl