Table 1.12.7.
pcDNA3-HA-MKK4 | 0 μg | 0.2 μg | 0.5 μg | 1.0 μg | 2.0 μg | 4.0 μg |
---|---|---|---|---|---|---|
pcDNA3 | 4.5 μg | 4.3 μg | 4.0 μg | 3.5 μg | 2.5 μg | 0.5 μg |
FuGENE HD | 13.5 μl | 13.5 μl | 13.5 μl | 13.5 μl | 13.5 μl | 13.5 μl |
Opti-MEM | 125 μl | 125 μl | 125 μl | 125 μl | 125 μl | 125 μl |