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Transfection to Determine MKK4- and Arrestin-3-Dependent JNK Activation
| pcDNA3-HA-MKK4 | 0 μg | 0.2 μg | 0.5 μg | 1.0 μg | 2.0 μg | 4.0 μg |
|---|---|---|---|---|---|---|
| pcDNA3 | 4.0 μg | 3.8 μg | 3.5 μg | 3.0 μg | 2.0 μg | 0 μg |
| pcDNA3-arrestin-3 | 0.5 μg | 0.5 μg | 0.5 μg | 0.5 μg | 0.5 μg | 0.5 μg |
| FuGENE HD | 13.5 μl | 13.5 μl | 13.5 μl | 13.5 μl | 13.5 μl | 13.5 μl |
| Opti-MEM | 125 μl | 125 μl | 125 μl | 125 μl | 125 μl | 125 μl |
