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. Author manuscript; available in PMC: 2024 Sep 1.
Published in final edited form as: Curr Protoc. 2023 Sep;3(9):e839. doi: 10.1002/cpz1.839

Table 2.12.8.1.

Transfection to evaluate the effect of T1A on JNK Activation by MKK4 (Figure 2.12.6 C) a

pcDNA3-HA-MKK4 0 μg 0.2 μg 0.5 μg 1.0 μg
pcDNA3 4.0 μg 3.8 μg 3.5 μg 3.0 μg
pcDNA-YFP 0 μg 0.2 μg 0 μg 0 μg
pcDNA-YFP 0 μg 0 μg 0.35 μg 0 μg
pcDNA3-arrestin-3 0 μg 0 μg 0 μg 0.5 μg
FuGENE HD 13.5 μl 13.5 μl 13.5 μl 13.5 μl
Opti-MEM 125 μl 125 μl 125 μl 125 μl
a

For MKK7 induced JNK activation, add pcDNA3-Flag-MKK7 instead of pcDNA3-HA-MKK4