FIG. 2.

Nucleotide sequence of the pls locus and deduced proteins. Promoter −35 and −10 sites, RBSs, and the transcriptional start (+1) are indicated by boldface letters. Vertical arrows indicate the cleavage sites in the α and β native peptides and the beginnings of the respective mature peptides. Direct repeats upstream of plsB are indicated by arrows. Sequences of dyad symmetry with the potential to serve as transcription terminators are indicated by arrows downstream of ORF2. Underlined amino acid sequences in ORF8 (plsK) indicate putative histidine (H), asparagine (N), and glycine (G) boxes, as defined by O’Connell-Motherway et al. (29). Stop codons are indicated by dashed lines at the ends of the protein sequences.