FIG. 3.

Northern and primer extension analysis of the pls operon. (A) Transcription product of the pls operon in an early-stationary-phase culture of L. plantarum LPCO10, obtained by using a 320-bp fragment containing part of the plsA and plsB genes as the probe. A total of 75 μg of RNA was loaded. Figures on the left indicate the sizes of the RNA standards used. (B) Primer extension analysis of plsB with oligonucleotide B1 (see text) as the primer. The arrow indicates the 84-nucleotide extension product generated. The size of the product was determined by comparison with the sequencing reaction products generated from pBluescript II SK(+) primed with the M13 (−20) universal primer which are shown in lanes A, C, G, and T.