| Antibody |
Anti-alpha actinin 2 (mouse monoclonal) |
Sigma |
A7811 |
IF, 1:200 |
| Antibody |
Anti-Titin (mouse monoclonal) |
DHSB |
9D10 |
IF, 1:2 |
| Antibody |
Mouse anti-Myomesin |
DHSB |
MYOM |
IF, 1:2 |
| Antibody |
Mouse anti-MYH |
DHSB |
A4.591 |
IF, 1:2 |
| Antibody |
Rabbit anti-MYH6 |
ProteinTech |
22281-1-AP |
WB, 1:500 |
| Antibody |
Rabbit anti-MYH7 |
ProteinTech |
22280-1-AP |
WB, 1:500 |
| Antibody |
Goat anti-mouse 488 |
Life Technologies |
A11001 |
IF, 1:100 |
| Antibody |
Goat anti-rabbit 488 |
Life Technologies |
A11034 |
IF, 1:100 |
| Antibody |
Goat anti-mouse 568 |
Life Technologies |
A11004 |
IF, 1:100 |
| Antibody |
Goat anti-rabbit 568 |
Life Technologies |
A11036 |
IF, 1:100 |
| Antibody |
Goat anti-mouse 647 |
Life Technologies |
A32728 |
IF, 1:100 |
| Antibody |
Goat anti-rabbit 647 |
Life Technologies |
A32733 |
IF, 1:100 |
| Biological sample (Bos taurus) |
Bovine serum albumin |
RPI |
A30075-100 |
|
| Chemical compound, drug |
Phalloidin Alexa Fluor 488 |
Invitrogen |
A12379 |
|
| Chemical compound, drug |
Phalloidin Alexa Fluor 568 |
Invitrogen |
A12380 |
|
| Chemical compound, drug |
Phalloidin Alexa Fluor 647 |
Invitrogen |
A22287 |
|
| Chemical compound, drug |
PBS, 10×, Ca2+/Mg2+ free |
Life Technologies |
70011-044 |
|
| Chemical compound, drug |
PBS, 10×, with Ca2+/Mg2+
|
Corning |
46-013CM |
|
| Chemical compound, drug |
PFA, 16% |
Electron Microscopy Sciences |
15710 |
|
| Chemical compound, drug |
0.5% Trypsin |
Life Technologies |
15400-054 |
|
| Chemical compound, drug |
0.1% Gelatin |
Sigma |
ES-006-B |
|
| Chemical compound, drug |
Dimethyl sulfoxide |
Sigma |
276855 |
|
| Chemical compound, drug |
Vectashield with DAPI |
Vector |
H-1200 |
|
| Chemical compound, drug |
Blebbistatin |
Sigma |
B0560 |
|
| Chemical compound, drug |
Fibronectin |
Corning |
354008 |
|
| Chemical compound, drug |
Lipofectamine RNAiMAX |
Thermo Fisher |
LMRNA015 |
|
| Chemical compound, drug |
TBS, 10× |
Corning |
46-012CM |
|
| Chemical compound, drug |
Tween 20 |
Sigma |
P7949 |
|
| Cell line (Homo sapiens) |
iCell cardiomyocytes^2 kit |
Fujifilm International |
CMC-100-012-000.5 |
|
| Sequence-based reagent |
SMART Pool siRNA against human MYH7 (13–16) |
Horizon Discovery |
A-011086 |
|
| Sequence-based reagent |
SMART Pool siRNA against human MYH7 (13, 14) |
Horizon Discovery |
A-011086-13, A-011086-14 |
|
| Sequence-based reagent |
siRNA against human MYH6 3’ UTR (13) |
Horizon Discovery |
A-012645-13-0005 |
|
| Sequence-based reagent |
siRNA against human MYH6 CDS (14) |
Horizon Discovery |
A-012645-14-0005 |
|
| Sequence-based reagent |
siRNA against human myomesin 3’UTR (13) |
Horizon Discovery |
A-006342-13-0005 |
|
| Sequence-based reagent |
siRNA against human myomesin CDS (16) |
Horizon Discovery |
A-006342-16-0005 |
|
| Software, algorithm |
FIJI |
NIH |
|
|
| Software, algorithm |
sarcApp |
This study |
|
|
| Software, algorithm |
yoU-Net |
This study |
|
|
