Extended Data Fig. 4. Effect of T-jump on average B-factor of refined apo and chitobiose-bound structures.
Models were refined against Laser Off, Experiment (apo or chitobiose bound), or Control structure factors. Controls exhibit similar B-factors across all time points, while B-factors for experimental measurements increase following T-jump. Apo models reveal a decline in B-factors at longer pump-probe time delays as complex motions develop, while chitobiose-bound experimental models retain higher B-factors at 200 μs, indicative of persistent, short-amplitude motions.