Fig. 3. Time-resolved difference electron density evolves over time following T-jump.
a, Comparison of weighted difference electron density maps (Ft – Fdark2) for each pump–probe time delay, centred around residues 97–100. Maps were visualized at an absolute contour level of ±0.04 e− Å−3 alongside corresponding refined models. While model coordinates appeared stable across pump–probe time delays, difference maps revealed time-resolved changes to the T-jump induced signal, with evidence for coordinated motions (green arrows) apparent by 200 µs. b, IADDAT was calculated as an average value per residue for each pump–probe time delay, then mapped onto C-alpha positions (spheres) of the respective model, and plotted as a function of residue number. c, Comparison of IADDAT values for experimental maps relative to matched controls revealed low levels of noise across the series.