Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2023 Nov 3;14:7060. doi: 10.1038/s41467-023-42809-y

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Fig. 2 — Representative images of anti-IBA1+ microglia, lipo-AF (excitation (Ex) 561 nm) and anti-CD68+ lysosomes in the developing (P5, P15, P30), young adult (P60, P90), and aged (24 months) gray matter (a, cortex) or white matter (d, corpus callosum). Scale bars = 10 μm. Quantification of the percentage of cortical microglial with negligible (0–0.1%), small (0.1–1%), moderate (1–2%) and high (>2%) percentages of their total volume occupied by lipo-AF in the gray (b) or white (e) matter. Quantification of the percentage of lipo-AF per field of view (FOV) inside microglia in the gray (c) and white (f) matter. c One-way ANOVA with Dunnett’s multiple comparisons test (F = 17.46, df = 22; P5 vs P90 p = 0.0020, P5 vs 24 m p < 0.0001). f One-way ANOVA with Dunnett’s multiple comparisons test (F = 7.225, df = 18; P15 vs 24 m p = 0.0012). g Quantification of the average percentage of microglial volume occupied by lipo-AF in gray and white matter. Two-way ANOVA with Šidák’s multiple comparisons test (Interaction: F = 1.877, df = 4; Row Factor: F = 190.5, df = 4; Column Factor: F = 23.81, df = 1; P60 p = 0.0058, P90 p = 0.0040). h Quantification of the average percentage of lipo-AF per field of view inside of microglia in gray and white matter microglia. Two-way ANOVA with Šidák’s multiple comparisons test (Interaction: F = 6.267, df = 4; Row Factor: F = 29.18, df = 4; Column Factor: F = 66.24, df = 1; P60 p = 0.0002, P90 & 24 m p < 0.0001); *p < 0.05 **p < 0.01 ***p < 0.001 ****p < 0.0001. All data are represented as mean ± SEM, n = 3–4 mice per experiment, 2 M, 2 F for each timepoint between P5-P90. 2 M, 1 F for 24 m. Source data are provided as a Source Data file.