Fig. 3. Lipo-AF in microglia during Alzheimer’s disease-related neurodegeneration.

a Representative images of anti-IBA1 immunolabelled microglia, Thioflavin-S labeled amyloid β plaques (ThioS), and lipo-AF in the somatosensory cortex of 9-month-old 5xFAD mice and wild-type (WT) littermates. Scale bars = 5 μm. b Quantification of total microglial volume occupied by lipo-AF in plaque-associated microglia, non-plaque associated microglia and microglia from WT littermates. Data are represented as mean ± SEM, n = 3 mice. One-way ANOVA with Tukey multiple comparisons test (F = 10.82, df = 8). c ThioS+ plaque volume correlated with total microglial lipo-AF volume normalized to microglial volume surrounding the plaque in the 5XFAD model. n = 61 individual plaques. Linear regression (F = 1.81; DFn = 1, DFd = 59; R² = 0.03105). (1 M, 2 F 5xFAD. 3 M, WT). Source data are provided as a Source Data file.