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. 2023 Nov 3;14:7062. doi: 10.1038/s41467-023-42440-x

Fig. 4. Inhaled aerosolized anti-SARS-CoV-2 mAb treatment prevents extensive lung tissue damage.

Fig. 4

a Top row shows photographs of lungs at necropsy for representative animals from each of the 5 treatment groups. The additional rows of micrographs show representative tissue sections stained with hematoxylin and eosin (H&E) at the sub-gross level with higher magnification depicted below. The control mAb pre-exposure group had acute interstitial pneumonia (Grade 4 affecting ~50% of tissue section) with lung lobes mottled light and dark pink with focally extensive pulmonary edema in caudal lobes (green arrows) and regional lymphadenopathy. SARS-CoV-2 mAb Pre-exposure group had minimal interstitial pneumonia (Grade 1 affecting ~10% of tissue section) with mildly mottled light and dark pink lung lobes and mild regional lymphadenopathy. Control mAb post-exposure group exhibited moderate to severe interstitial pneumonia (Grade 4 affecting up to 50% of tissue section) with multiple foci of non-collapsing parenchyma most commonly observed in the caudal lung lobes (green arrow). SARS-CoV-2 mAb post-exposure group had minimal to moderate interstitial pneumonia (Grade 3 affecting ~10% of tissue section) with mottled light and dark pink areas present most abundantly in the caudal lung lobes (green arrow) with regional lymphadenomegaly. SARS-CoV-2 mAb Pre/Post-exposure group had minimal to mild interstitial pneumonia (Grade 2 affecting ~10% of tissue section) with mottled light and dark pink lung lobes that affected the caudal lung lobes primarily. Scale bar = 5 mm in middle panel photomicrographs except for the SARS-CoV-2 Pre-exposure group, which = 4 mm. Scale bar in bottom panel = 200 µm. b and c H&E staining of lung sections. Scale bars = 4 mm and 200 µm in the upper and lower images, respectively. b Necrotizing, fibrinous hemorrhagic pneumonia detected in over 50% of the tissue section from animal 38418 (control mAb pre-exposure). c Animal 38579 (SARS-CoV-2 mAb post-exposure), demonstrated necrotizing, fibrinous hemorrhagic pneumonia affecting ~10% of tissue section. d Semiquantitative scoring system was developed by assessing the interstitial cellularity of the alveolar septa as illustrated in Supplementary Fig 4. Individual data points for each animal (n = 4 animal per group) within each group are shown as the mean and SEM for each group.