Table 1.

Antibody concentrations and nebulization times

Treatment group	Animal ID	Nebulization date	Antibody amount (mg)	Antibody volume (mL)	Animal weight (kg)	Antibody concentration (mg/mL)	Nebulization Time
Control Pre-exposure	38418 38901 38956 38958	4/11/22 4/11/22 4/12/22 4/12/22	60 60 60 60	4.5 4.5 4.5 4.5	4.75 4.70 4.05 4.80	13.3 13.3 13.3 13.3	7 min 55 s 8 min 33 s Not recorded Not recorded
SARS-CoV-2 Pre-exposure	38567 38939 38907 39055	4/20/22 4/20/22 4/21/22 4/21/22	60 60 60 60	2.5 2.5 2.5 2.5	5.25 4.95 5.50 4.60	24 24 24 24	6 min 20 s 5 min 30 s 5 min 6 s 6 min 22 s
Control Post-exposure	39057 39078 38957 38960	4/29/22 4/29/22 4/30/22 4/30/22	60 60 60 60	4.5 4.5 4.5 4.5	3.65 4.25 4.85 4.10	13.3 13.3 13.3 13.3	~8 min ~8 min Not recorded Not recorded
SARS-CoV-2 Post-exposure	37744 38579 38469 38941	6/2/22 6/3/22 6/2/22 6/3/22 6/3/22 6/4/22 6/3/22 6/4/22	60 60 60 60 60 60 60 60	3.0 3.0 3.0 3.0 3.0 3.0 3.0 3.0	4.90 4.90 4.15 3.85 3.70 3.55 3.90 3.90	20 20 20 20 20 20 20 20	9 min 10 min 10 min 9 min 6 min 6 min 6 min 12 s 6 min 37 s
SARS-CoV-2 Pre/Post- exposure	38323 38885 39007 38929	5/16/22 5/17/22 5/18/22 5/16/22 5/17/22 5/18/22 5/17/22 5/18/22 5/19/22 5/17/22 5/18/22 5/19/22	60 60 60 60 60 60 60 60 60 60 60 60	3.0 3.0 3.0 3.0 3.0 3.0 3.0 3.0 3.0 3.0 3.0 3.0	5.05 5.05 4.95 4.05 3.80 3.75 4.25 4.15 4.15 4.95 4.80 4.75	20 20 20 20 20 20 20 20 20 20 20 20	5 min 10 s 5 min 30 s 5 min 35 s 5 min 30 s 5 min 33 s 6 min 12 s 8 min 30 s 8 min 25 s 10 min 7 min 10 s 7 min 55 s 8 min 30 s

All nebulizations were performed with 2 L/min oxygen flow rate. Liquid holdup was not measured but is estimated to be <0.5 mL.